ASTM E1153

Efficacy of Sanitizers Recommended for Inanimate, Hard, Non-porous, Non-Food Contact Sanitizers 

Summary

The Efficacy of Sanitizers Recommended for Inanimate, Hard, Non-porous, Non-Food Contact Sanitizers test is a quantitative evaluation designed to assess disinfectant substances’ ability to treat non-food contact surfaces for submission to the Environmental Protection Agency. The typical challenge microorganisms are Staphylococcus aureus, Klebsiella pneumoniae or Klebsiella aerogenes. 

Test Overview

• Five replicates, per lot of test substance, are evaluated using three lots of a single substance at one concentration. 
• Challenge microorganisms are dried on square carriers of the desired surface type (for example, glass or ceramic) and exposed to the test substance according to the Sponsor’s directions, held for the stipulated contact time, neutralized and then vigorously mixed. 
• Dilutions of the neutralized test substance are plated, incubated, and observed for growth.
• Parallel (untreated) controls are run concurrently with the test samples.

Parallel controls allow a percent reduction to be calculated for the test carriers. The test substance demonstrates efficacy if the test results exhibit a bacterial reduction of at least 99.9% over the parallel control within five minutes.