Cyclospora Testing


Why is Cyclospora testing important?

Cyclospora cayetanensis is the pathogenic species of Cyclospora, and a microscopicparasite responsible for cyclosporiasis. Humans can become infected by consuming food or water contaminated with the parasite. Symptoms include diarrhea, cramping, bloating, fever, nausea, and fatigue and can take 2 days to 2 weeks to develop and can last several weeks to a month if not longer. The infection is generally not life threatening but can return and continue long after infection has occurred. The contamination occurs usually from water sources containing stool from infected humans as no known animal reservoirs of the parasite are known. Usually leafy green herbs are the common contaminated foods but berries and lettuces are also high risk matrixes.

The FDA has recently released an action plan in response to the increased outbreaks and recalls in produce due to Cyclospora cayetanensis contamination. This plan, linked below, is based off similar plans to address STEC (Shiga toxin producing E.coli) contamination in produce, and lays out plans for prevention, response, and education. This plan included increased surveillance and internal sampling and development of new testing methods and kits.

View the FDA Action Plan

Why Partner with Microbac

Microbac is one of the few labs with an accredited method based off FDA guidance.
Microbac has a long history of responding to emerging testing and public health needs
Microbac prides itself on working with our clients and being flexible on their needs

Need Cyclospora Testing?

Request Testing

Sample Submission and Testing Process

Contact Microbac to discuss your testing needs and set up an account
Submit the sample to your local lab by cold chain, only after your account is established
Sample is processed, and analysis ran which targets DNA of Cyclospora
Once testing is completed and data & controls reviewed, the client will receive a certificate of analysis with results

How is product tested

Microbac utilizes the FDA BAM Chapter 19B method for testing utilizing Real-time polymerase chain reaction (RT-PCR) technology. A basic walkthrough of the method is listed below:


Sample portion is washed used using specialized buffer solution.


Buffer is separated from sample and centrifuged to separate target from other material in buffer.


Buffer is transferred to microtube along with target primers and loaded into equipment.


If target DNA is present the qPCR cycling reaction releases fluorophores resulting in a signal read by the instrument.


A change in measurements during the process indicates a positive result. Positive and Negative controls are run side by side to ensure high quality of data.

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